Application
Antibody is used in Western blot to detect TrxL in HL-60 cell lysate.Application
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.| Product Name | TXNL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Thioredoxin-like protein 1, 32 kDa thioredoxin-related protein, TXNL1, TRP32, TXL, TXNL |
| Product description | Thioredoxins are a group of small redox active proteins possessing a conserved active site sequence. TrxL (Thioredoxin like protein 1) has 2 distinct domains: an N terminal domain, which is 43% identical to human TXN, and a C terminal domain, which shows no homology to other proteins in the sequence databases. The active site sequence, located within the N terminal domain, is that of a thioredoxin like protein; compared to the active site sequence of TXN, it has a single amino acid substitution. Unlike other thioredoxin like proteins, TrxL does not serve as a substrate for thioredoxin reductase in an insulin assay.1) Miranda-Vizuete, A., et al., Biochem. Biophys. Res. Commun. 243: 284-288 (1998). |
| Immunogen | This TXNL1 (TRP32/TrxL) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-289 amino acids from the C-terminal region of human TXNL1 (TRP32/TrxL). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TXNL1 |
|---|---|
| Alternative Names | Thioredoxin-like protein 1 32 kDa thioredoxin-related protein TXNL1 TRP32 TXL TXNL |
| Molecular Weight(MW) | 32 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Antibody is used in Western blot to detect TrxL in HL-60 cell lysate.
Application
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. Trxl-1 antibody gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
