Application
Western blot analysis of TYRO10 using rabbit polyclonal TYRO10 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2).Application
Western blot analysis of TYRO10 Antibody in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TYRO10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosin |
| Product description | Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane and cytoplasmic regions. There are several subclasses of RTKs and TYRO10 belongs to a novel subclass. The deduced amino acid sequence of TYRO10 has a unique extracellular region encompassing a factor VIII-like domain, not previously described for RTKs.1) Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). |
| Immunogen | This TYRO10 antibody is generated from rabbits immunized with a his tag recombinant protein of human TYRO10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DDR2 |
|---|---|
| Alternative Names | Discoidin domain-containing receptor 2 Discoidin domain receptor 2 CD167 antigen-like family member B Discoidin domain-containing receptor tyrosine kinase 2 Neurotrophic tyrosine kinase receptor-related 3 Receptor protein-tyrosine kinase TKT Tyrosin |
| Molecular Weight(MW) | 97 kDa |
Application
Western blot analysis of TYRO10 using rabbit polyclonal TYRO10 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2).
Application
Western blot analysis of TYRO10 Antibody in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
