Application
Immunohistochemistry staining of Tyrosine Hydroxylase in adrenal tissue using Tyrosine Hydroxylase Antibody.Application
Western Blot analysis of Tyrosine Hydroxylase recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific using Tyrosine Hydroxylase Antibody.| Product Name | Tyrosine Hydroxylase (phospho Ser40) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase |
| Product description | Tyrosine hydroxylase in involved in the conversion of phenylalanine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines and as such has a key role in the physiology of adrenergic neurons. TH is encoded by four distinct mRNAs produced by alternative splicing of a single primary transcript. Expression of the mRNAs varies in different parts of the nervous system. Defects in TH are the cause of autosomal recessive Segawa syndrome. |
| Immunogen | Tyrosine Hydroxylase antibody was raised against a phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of Tyrosine Hydroxylase (Rat). |
| Modification | p-Ser40 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Tyrosine Hydroxylase antibody can be used in ELISA, Western Blot starting at 1:1000 - 1:3000, and immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TH |
|---|---|
| Alternative Names | TH DYT14 Dystonia 14 TYH Tyrosine 3-hydroxylase Tyrosine hydroxylase DYT5b Tyrosine 3-monooxygenase |
| Tissue Specificity | Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. |
Application
Immunohistochemistry staining of Tyrosine Hydroxylase in adrenal tissue using Tyrosine Hydroxylase Antibody.
Application
Western Blot analysis of Tyrosine Hydroxylase recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific using Tyrosine Hydroxylase Antibody.| Application Notes | Tyrosine Hydroxylase antibody can be used in ELISA, Western Blot starting at 1:1000 - 1:3000, and immunohistochemistry starting at 10 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store Tyrosine Hydroxylase antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 50 uL of 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50% glycerol. |
