Application
Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/mL.Application
Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 μg/mL.| Product Name | TYW1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYW1 Antibody: TYW1A, RSAFD1, YPL207W, S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, Radical S-adenosyl methionine and flavodoxin domain-containing protein 1 |
| Product description | TYW1 Antibody: TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions1) Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6 |
| Immunogen | TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1. The immunogen is located within amino acids 520 - 570 of TYW1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TYW1 |
|---|---|
| Alternative Names | TYW1 Antibody: TYW1A RSAFD1 YPL207W S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase Radical S-adenosyl methionine and flavodoxin domain-containing protein 1 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms. |
Application
Western blot analysis of TYW1 in human lung tissue lysate with TYW1 antibody at 1 μg/mL.
Application
Immunohistochemistry of TYW1 in human lung tissue with TYW1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
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| Application Notes | TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TYW1 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TYW1 Antibody is supplied in PBS containing 0.02% sodium azide. |
