Application
Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL.| Product Name | TYW2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYW2 Antibody: TYW2, TRM12, TYW2, tRNA wybutosine-synthesizing protein 2 homolog, Alpha-amino-alpha-carboxypropyl transferase TYW2, tRNA-yW-synthesizing protein 2 |
| Product description | TYW2 Antibody: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.1) Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6 |
| Immunogen | TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRMT12 |
|---|---|
| Alternative Names | TYW2 Antibody: TYW2 TRM12 TYW2 tRNA wybutosine-synthesizing protein 2 homolog Alpha-amino-alpha-carboxypropyl transferase TYW2 tRNA-yW-synthesizing protein 2 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms |
Application
Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
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| Application Notes | TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TYW2 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TYW2 Antibody is supplied in PBS containing 0.02% sodium azide. |
