Application
Western blot analysis of TYW3 in A549 cell lysate with TYW3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | TYW3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYW3 Antibody: C1orf171, C1orf171, tRNA wybutosine-synthesizing protein 3 homolog, tRNA-yW-synthesizing protein 3 |
| Product description | TYW3 Antibody: TYW3 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.1) Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6. |
| Immunogen | TYW3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TYW3. The immunogen is located within amino acids 160 - 210 of TYW3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TYW3 antibody can be used for detection of TYW3 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TYW3 |
|---|---|
| Alternative Names | TYW3 Antibody: C1orf171 C1orf171 tRNA wybutosine-synthesizing protein 3 homolog tRNA-yW-synthesizing protein 3 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (94%) |
| Tissue Specificity | TYW3 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW3 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of TYW3 in A549 cell lysate with TYW3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | TYW3 antibody can be used for detection of TYW3 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TYW3 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TYW3 Antibody is supplied in PBS containing 0.02% sodium azide. |
