Application
Western blot analysis of TYW4 in rat brain tissue lysate with TYW4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TYW1 in mouse brain tissue with TYW1 antibody at 20 μg/mL.Application
Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 μg/mL.| Product Name | TYW4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYW4 Antibody: PPM2, TYW4, KIAA0547, tRNA wybutosine-synthesizing protein 4, Leucine carboxyl methyltransferase 2, tRNA yW-synthesizing protein 4 |
| Product description | TYW4 Antibody: TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.1) Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6. |
| Immunogen | TYW4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4. The immunogen is located within the first 50 amino acids of TYW4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TYW4 antibody can be used for detection of TYW4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LCMT2 |
|---|---|
| Alternative Names | TYW4 Antibody: PPM2 TYW4 KIAA0547 tRNA wybutosine-synthesizing protein 4 Leucine carboxyl methyltransferase 2 tRNA yW-synthesizing protein 4 |
| Tissue Specificity | TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform. |
Application
Western blot analysis of TYW4 in rat brain tissue lysate with TYW4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TYW1 in mouse brain tissue with TYW1 antibody at 20 μg/mL.
Application
Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | TYW4 antibody can be used for detection of TYW4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TYW4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TYW4 Antibody is supplied in PBS containing 0.02% sodium azide. |
