Application
Western blot analysis of anti- in Ramos cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.| Product Name | U2AF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP |
| Product description | U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.1) Rino,J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little,J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco,T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006) |
| Immunogen | This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | U2AF1 |
|---|---|
| Alternative Names | Splicing factor U2AF 35 kDa subunit U2 auxiliary factor 35 kDa subunit U2 small nuclear RNA auxiliary factor 1 U2 snRNP auxiliary factor small subunit U2AF1 U2AF35 U2AFBP |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of anti- in Ramos cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of U2AF1 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
