Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | UBE2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2C, UBCH10, dJ447F3.2 |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.1) Ieta, K., (2007) Int. J. Cancer 121 (1), 33-38. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2C. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UBE2C antibody can be used for detection of UBE2C by ELISA at 1:62500. UBE2C antibody can be used for detection of UBE2C by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2C |
|---|---|
| Alternative Names | UBE2C UBCH10 dJ447F3.2 |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | UBE2C antibody can be used for detection of UBE2C by ELISA at 1:62500. UBE2C antibody can be used for detection of UBE2C by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2C antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
