Application
Antibody is used in Western blot to detect UBE2C in Jurkat cell lysate.Application
Western blot analysis of UBE2C using rabbit polyclonal UBE2C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2).| Product Name | UBE2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-conjugating enzyme E2 C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C, UBE2C, UBCH10 |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.1) Okamoto, Y., et al., Cancer Res. 63(14):4167-4173 (2003). |
| Immunogen | This UBE2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-172 amino acids from the C-terminal region of human UBE2C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | UBE2C |
|---|---|
| Alternative Names | Ubiquitin-conjugating enzyme E2 C UbcH10 Ubiquitin carrier protein C Ubiquitin-protein ligase C UBE2C UBCH10 |
| Molecular Weight(MW) | 20 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Antibody is used in Western blot to detect UBE2C in Jurkat cell lysate.
Application
Western blot analysis of UBE2C using rabbit polyclonal UBE2C Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2C gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
