Application
Western blot analysis in Hela cell line lysates (35ug/lane).Application
Western blot analysis of UBE2D3 using rabbit polyclonal UBE2D3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.| Product Name | UBE2D3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-conjugating enzyme E2 D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3, UBE2D3, UBC5C, UBCH5C |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.1) Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010) |
| Immunogen | This UBE2D3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-136 amino acids from the C-terminal region of human UBE2D3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2D3 |
|---|---|
| Alternative Names | Ubiquitin-conjugating enzyme E2 D3 Ubiquitin carrier protein D3 Ubiquitin-conjugating enzyme E2(17)KB 3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-protein ligase D3 UBE2D3 UBC5C UBCH5C |
| Molecular Weight(MW) | 17 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Western blot analysis in Hela cell line lysates (35ug/lane).
Application
Western blot analysis of UBE2D3 using rabbit polyclonal UBE2D3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2D3 gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
