Application
Antibody used in IHC on Human Intestine.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | UBE2J2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2J2, NCUBE2, PRO2121, NCUBE-2 |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.1) Otsuki, T., (2005) DNA Res. 12 (2), 117-126. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2J2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| UBE2J2 antibody can be used for detection of UBE2J2 by ELISA at 1:312500. UBE2J2 antibody can be used for detection of UBE2J2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UBE2J2 |
|---|---|
| Alternative Names | UBE2J2 NCUBE2 PRO2121 NCUBE-2 |
| Molecular Weight(MW) | 30 kDa, 29 kDa, 23 kDa, 23 kDa |
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | UBE2J2 antibody can be used for detection of UBE2J2 by ELISA at 1:312500. UBE2J2 antibody can be used for detection of UBE2J2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2J2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
