Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.
The covalent conjugation of ubiquitin to proteins
regulates diverse cellular pathways and proteins. Ubiquitin is
transferred to a target protein through a concerted action of a
ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme
(E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al.,
2006 [PubMed 16916645]).1) Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)
Immunogen
This UBE2T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the C-terminal region of human UBE2T.
Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.