Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | UBE3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE3B, DKFZp586K2123, DKFZp686A1051, FLJ45294, MGC131858, MGC78388, BPIDS |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE3B is a member of the E3 ubiquitin-conjugating enzyme family. UBE3B may interact with other proteins and play a role in stress response.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family. The encoded protein may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.1) Szafranski, K., Genome Biol. 8 (8), R154 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE3B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UBE3B antibody can be used for detection of UBE3B by ELISA at 1:312500. UBE3B antibody can be used for detection of UBE3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE3B |
|---|---|
| Alternative Names | UBE3B DKFZp586K2123 DKFZp686A1051 FLJ45294 MGC131858 MGC78388 BPIDS |
| Molecular Weight(MW) | 123 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | UBE3B antibody can be used for detection of UBE3B by ELISA at 1:312500. UBE3B antibody can be used for detection of UBE3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
