Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Below: Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.| Product Name | Ubiquitin Hydrolase 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDGOA, PARK5, PGP95, PGP9.5, Uch-L1, HEL-117, PGP 9.5, Ubiquitin thioesterase L1, UCH-L1 |
| Product description | Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.1) Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983). |
| Immunogen | Recombinant full length human UCHL1 purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | UCHL1 |
|---|---|
| Alternative Names | NDGOA PARK5 PGP95 PGP9.5 Uch-L1 HEL-117 PGP 9.5 Ubiquitin thioesterase L1 UCH-L1 |
| Molecular Weight(MW) | 24 |
| Tissue Specificity | Specific for the ~24kDa UCHL1 protein. |
Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. Below: Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Ubiquitin Hydrolase 1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
