Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | UBTF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1 |
| Product description | Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).1) Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010) |
| Immunogen | This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBTF |
|---|---|
| Alternative Names | Nucleolar transcription factor 1 Autoantigen NOR-90 Upstream-binding factor 1 UBF-1 UBTF UBF UBF1 |
| Molecular Weight(MW) | 89 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
