Application
Western blot analysis of UCMA in SW1353 cell lysate with UCMA antibody at 2.5 μg/mL.| Product Name | UCMA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCMA Antibody: GRP, C10orf49, Unique cartilage matrix-associated protein, Gla-rich protein, Ucma-C |
| Product description | UCMA Antibody: UCMA is a secreted cartilage-specific protein that was discovered in a screen for differentially expressed genes in retinoic acid-treated mouse chondrocytes. It was also identified in a human chondrocyte EST screen for candidate genes of skeletal dysplasias. UCMA expression is thought to parallel that of collagen II with its expression decreasing with maturation chrondrocytes mature. UCMA is processed by a furin-like protease into two fragments, an amino-terminal fragment and a carboxy-terminal fragment (UCMA-C). Application of recombinant UCMA-C to primary osteoblasts, mesenchymal stem cells, and MC3T3-E1 pre-osteoblasts interferes with their osteogenic differentiation, but does not affect expression of chondrocyte-specific genes or chondrocyte proliferation, suggesting that UCMA may be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells. At least two isoforms of UCMA are known to exist.1) Surmann-Schmitt C, Dietz U, Kireva T, et al. Ucma, a novel secreted cartilage-specific protein with implications in osteogenesis. J. Biol. Chem.2008; 283:7082-93. |
| Immunogen | UCMA antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human UCMA. The immunogen is located within the last 50 amino acids of UCMA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UCMA |
|---|---|
| Alternative Names | UCMA Antibody: GRP C10orf49 Unique cartilage matrix-associated protein Gla-rich protein Ucma-C |
| Molecular Weight(MW) | Predicted: 15 kDa Observed: 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (81%) |
Application
Western blot analysis of UCMA in SW1353 cell lysate with UCMA antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1214 - SW1353 Cell Lysate |
|---|---|
| Application Notes | UCMA antibody can be used for detection of UCMA by Western blot at 2.5 - 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UCMA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | UCMA Antibody is supplied in PBS containing 0.02% sodium azide. |
