UGCGL2 recognizes glycoproteins with minor folding defects. UGCGL2 reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.1) Arnold, S.M. (2003) J. Biol. Chem. 278 (44), 43320-43328.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UGCGL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
UGCGL2 antibody can be used for detection of UGCGL2 by ELISA at 1:12500. UGCGL2 antibody can be used for detection of UGCGL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
UGCGL2 antibody can be used for detection of UGCGL2 by ELISA at 1:12500. UGCGL2 antibody can be used for detection of UGCGL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store UGCGL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.