Application
Western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).Application
UGT2B15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of UGT2B15 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UGT2B15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UDP-glucuronosyltransferase 2B15, UDPGT 2B15, HLUG4, UDP-glucuronosyltransferase 2B8, UDPGT 2B8, UDPGTh-3, UGT2B15, UGT2B8 |
| Product description | The UGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. UGT2B8 demonstrates reactivity with estriol. See UGT2B4 (MIM 600067).1) Yong, M., et al. Cancer Epidemiol. Biomarkers Prev. 19(2):537-546(2010) |
| Immunogen | This UGT2B15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human UGT2B15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UGT2B15 |
|---|---|
| Alternative Names | UDP-glucuronosyltransferase 2B15 UDPGT 2B15 HLUG4 UDP-glucuronosyltransferase 2B8 UDPGT 2B8 UDPGTh-3 UGT2B15 UGT2B8 |
| Molecular Weight(MW) | 61 kDa |
Application
Western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).
Application
UGT2B15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of UGT2B15 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
