UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Immunogen
This UGT2B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human UGT2B4.
Western Blot at 1:8000 dilution Lane 1: human liver lysate Lane 2: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.