Application
Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human heart lysates Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).Application
UGT8 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | UGT8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase, UDP-galactose-ceramide galactosyltransferase, UGT8, CGT, UGT4 |
| Product description | Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.[supplied by OMIM].1) Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010) |
| Immunogen | This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UGT8 |
|---|---|
| Alternative Names | 2-hydroxyacylsphingosine 1-beta-galactosyltransferase Ceramide UDP-galactosyltransferase Cerebroside synthase UDP-galactose-ceramide galactosyltransferase UGT8 CGT UGT4 |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human heart lysates Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Application
UGT8 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
