Application
Western blot analysis of UHRF1BP1 in mouse kidney tissue lysate with UHRF1BP1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 20 μg/mL.Application
Immunohistochemistry of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 2.5 μg/mL.| Product Name | UHRF1BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UHRF1BP1 Antibody: ICBP90, C6orf107, dJ349A12.1, UHRF1-binding protein 1, ICBP90-binding protein 1 |
| Product description | UHRF1BP1 Antibody: The Ubiquitin-like containing PHD and RING finger domains 1-binding protein 1 (UHRF1BP1), also known as ICBP90, is a transcription and cell cycle regulator. It specifically binds to the histone H3 N-terminal tail when methylated on K9 by two functional domains, a PHD finger that defines the binding specificity and an SRA (SET- and RING-associated) domain that promotes binding activity. UHRF1BP1 is required for proper heterochromatin formation in mammalian cells. Furthermore, UHRF1BP1 is thought to be a pivotal target for the ERK1/2 signaling pathway to control the proliferation of Jurkat T cells.1) Hopfner R, Mousli M, Jeltsch JM, et al. ICBP90, a novel human CCAAT binding protein, involved in the regulation of Topoisomerase IIα expression. Cancer Res. 2000; 60:121-8 |
| Immunogen | UHRF1BP1 antibody was raised against a 17 amino acid synthetic peptide near the center of human UHRF1BP1. The immunogen is located within amino acids 890 - 940 of UHRF1BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| UHRF1BP1 antibody can be used for detection of UHRF1BP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UHRF1BP1 |
|---|---|
| Alternative Names | UHRF1BP1 Antibody: ICBP90 C6orf107 dJ349A12.1 UHRF1-binding protein 1 ICBP90-binding protein 1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | UHRF1BP1 antibody is predicted to not cross-react with UHRF1BP1L |
Application
Western blot analysis of UHRF1BP1 in mouse kidney tissue lysate with UHRF1BP1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 20 μg/mL.
Application
Immunohistochemistry of UHRF1BP1 in human kidney tissue with UHRF1BP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | UHRF1BP1 antibody can be used for detection of UHRF1BP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UHRF1BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | UHRF1BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
