Application
Western blot analysis of Unc93b in human heart tissue lysate with Unc93b antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of Unc93b in Human Heart cells with Unc93b antibody at 20 μg/mL.Application
Immunohistochemistry of Unc93b in human heart with Unc93b antibody at 2.5 μg/mL.| Product Name | Unc93b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Unc93b Antibody: IIAE1, UNC93, UNC93B, Unc-93B1, Protein unc-93 homolog B1 |
| Product description | Unc93b Antibody: The endoplasmic reticulum (ER) protein Unc93b, a human homolog of the C. elegans Unc93 gene, was initially identified by a forward genetic screen using N-ethyl-N-nitrosourea where a histidine-to-arginine substitution in Unc93b caused defects in Toll-like receptor (TLR) 3, 7 and 9 signaling. Unlike Unc93a, another homolog of the C. elegans Unc93 gene whose function is unknown, Unc93b specifically interacts with TLR3, 7 and 9; the histidine-to-arginine point mutation used to identify Unc93b abolishes this interaction. Mice carrying this point mutation are highly susceptible to infection with a number of viruses, indicating that Unc93b plays an important role in innate immunity.1) Tabeta K, Hoebe K, Janssen EM, et al. The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7, and 9. Nat. Immunol. 2006; 7:156-64. |
| Immunogen | Unc93b antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Unc93b. The immunogen is located within amino acids 80 - 130 of Unc93b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Unc93b antibody can be used for the detection of Unc93b by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UNC93B1 |
|---|---|
| Alternative Names | Unc93b Antibody: IIAE1 UNC93 UNC93B Unc-93B1 Protein unc-93 homolog B1 |
| Molecular Weight(MW) | 56, 66 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | Multiple isoforms of Unc93b are known to exist. This antibody will not cross-react with Unc93a. |
Application
Western blot analysis of Unc93b in human heart tissue lysate with Unc93b antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of Unc93b in Human Heart cells with Unc93b antibody at 20 μg/mL.
Application
Immunohistochemistry of Unc93b in human heart with Unc93b antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | Unc93b antibody can be used for the detection of Unc93b by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Unc93b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Unc93b Antibody is supplied in PBS containing 0.02% sodium azide. |
