Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UPF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Regulator of nonsense transcripts 2, Nonsense mRNA reducing factor 2, Up-frameshift suppressor 2 homolog, hUpf2, UPF2, KIAA1408, RENT2 |
| Product description | UPF2 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p.1) Clerici, M., et al. EMBO J. 28(15):2293-2306(2009) |
| Immunogen | This UPF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 630-656 amino acids from the Central region of human UPF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UPF2 |
|---|---|
| Alternative Names | Regulator of nonsense transcripts 2 Nonsense mRNA reducing factor 2 Up-frameshift suppressor 2 homolog hUpf2 UPF2 KIAA1408 RENT2 |
| Molecular Weight(MW) | 148 kDa |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
