Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Product Name | UPF3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPF3B, HUPF3B, MRXS14, RENT3B, UPF3X, MRX62 |
| Product description | UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.1) Chamieh, H., (2008) Nat. Struct. Mol. Biol. 15 (1), 85-93. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UPF3B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UPF3B antibody can be used for detection of UPF3B by ELISA at 1:312500. UPF3B antibody can be used for detection of UPF3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UPF3B |
|---|---|
| Alternative Names | UPF3B HUPF3B MRXS14 RENT3B UPF3X MRX62 |
| Molecular Weight(MW) | 58 kDa |
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | UPF3B antibody can be used for detection of UPF3B by ELISA at 1:312500. UPF3B antibody can be used for detection of UPF3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UPF3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
