Application
Western blot analysis of UPK1B in human kidney tissue lysate with UPK1B antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.Application
Immunofluorescence of UPK1B in human bladder muscle tissue with UPK1B antibody at 20 μg/mL.Application
Immunohistochemistry of UPK1B in human bladder tissue with UPK1B antibody at 5 μg/mL.| Product Name | UPK1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPK1B Antibody: UPIB, UPK1, TSPAN20, Uroplakin-1b, Tetraspanin-20, UP1b |
| Product description | UPK1B Antibody: Uroplakin-1B (UPK1B) is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. UPK1B is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins and may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.1) Yu J, Lin JH, Wu XR, et al. Uroplakins Ia and Ib, two major differentiation products of bladder epithelium, belong to a family of four transmembrane domain (4TM) proteins. J. Cell Biol. 1994; 125:171-82. |
| Immunogen | UPK1B antibody was raised against a 17 amino acid synthetic peptide near the center of human UPK1B. The immunogen is located within amino acids 110 - 160 of UPK1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| UPK1B antibody can be used for detection of UPK1B by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UPK1B |
|---|---|
| Alternative Names | UPK1B Antibody: UPIB UPK1 TSPAN20 Uroplakin-1b Tetraspanin-20 UP1b |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | At least two isoforms of UPK1B are known to exist; this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members. |
Application
Western blot analysis of UPK1B in human kidney tissue lysate with UPK1B antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
Application
Immunofluorescence of UPK1B in human bladder muscle tissue with UPK1B antibody at 20 μg/mL.
Application
Immunohistochemistry of UPK1B in human bladder tissue with UPK1B antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | UPK1B antibody can be used for detection of UPK1B by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UPK1B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | UPK1B Antibody is supplied in PBS containing 0.02% sodium azide. |
