Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).Application
USO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | USO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP |
| Product description | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.1) Striegl, H., et al. PLoS ONE(2), E8991 (2010) : |
| Immunogen | This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | USO1 |
|---|---|
| Alternative Names | General vesicular transport factor p115 Protein USO1 homolog Transcytosis-associated protein TAP Vesicle-docking protein USO1 VDP |
| Molecular Weight(MW) | 108 kDa |
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Application
USO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
