Application
Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.Application
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.| Product Name | USP10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USP10 Antibody: UBPO, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10 |
| Product description | USP10 Antibody: USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.1) D’Andrea A and Pellman D. Deubiquitinating enzymes: a new class of biological regulators. Crit. Rev. Biochem. Mol. Biol.1998; 33:337-52. |
| Immunogen | USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10. The immunogen is located within amino acids 30 - 80 of USP10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | USP10 |
|---|---|
| Alternative Names | USP10 Antibody: UBPO KIAA0190 Ubiquitin carboxyl-terminal hydrolase 10 Deubiquitinating enzyme 10 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
Application
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | USP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | USP10 Antibody is supplied in PBS containing 0.02% sodium azide. |
