WB
Western blot analysis of anti-USP10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-USP10 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)ICC/IF
Anti-USP10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10 .FC
Flow cytometric Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).| Product Name | Mouse monoclonal antibody to USP10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|
| Species Reactivity | DogHumanMonkeyMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A/G |
| Gene Symbol | USP10 |
|---|---|
| Gene Synonyms | UBPO |
| Gene Full Name | ubiquitin specific peptidase 10 |
| Gene Summary | Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013] |
| Alternative Names | USP10 KIAA0190 Ubiquitin carboxyl-terminal hydrolase 10 Deubiquitinating enzyme 10 |
| Molecular Weight(MW) | 87 kDa(Observed band size:110 kDa) |
| Cellular Localization | Cytoplasm,Endosome,Nucleus. |
WB
Western blot analysis of anti-USP10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-USP10 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
ICC/IF
Anti-USP10 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10 .
FC
Flow cytometric Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for 12 months from date of receipt. Store at -20°C as received. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
