USP16 is a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human USP16.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
USP16 antibody can be used for detection of USP16 by ELISA at 1:12500. USP16 antibody can be used for detection of USP16 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human brain stems (NT2) at 1:500.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
USP16 antibody can be used for detection of USP16 by ELISA at 1:12500. USP16 antibody can be used for detection of USP16 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store USP16 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt