Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Product Name | UTX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UTX, DKFZp686A03225, MGC141941, bA386N14.2, UTX, KABUK2 |
| Product description | UTX is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. UTX plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.1) Hong, S., (2007) Proc. Natl. Acad. Sci. U.S.A. 104 (47), 18439-18444. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UTX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| UTX antibody can be used for detection of UTX by ELISA at 1:312500. UTX antibody can be used for detection of UTX by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDM6A |
|---|---|
| Alternative Names | UTX DKFZp686A03225 MGC141941 bA386N14.2 UTX KABUK2 |
| Molecular Weight(MW) | 154 kDa |
Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | UTX antibody can be used for detection of UTX by ELISA at 1:312500. UTX antibody can be used for detection of UTX by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UTX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM294794