Application
Western blot analysis of UVRAG in mouse lung tissue lysate with UVRAG antibody at 1 μg/ml.Application
Immunofluorescence of UVRAG in human lung tissue with UVRAG antibody at 20 μg/ml.Application
Immunohistochemistry of UVRAG in human lung tissue with UVRAG antibody at 5 μg/ml.| Product Name | UVRAG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UVRAG Antibody: p63, DHTX, VPS38, UV radiation resistance-associated gene protein, p63 |
| Product description | The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2). UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).1) Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34. |
| Immunogen | UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG. The immunogen is located within amino acids 490 - 540 of UVRAG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UVRAG |
|---|---|
| Alternative Names | UVRAG Antibody: p63 DHTX VPS38 UV radiation resistance-associated gene protein p63 |
| Molecular Weight(MW) | Predicted: 77 kDa Observed: 75 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | UVRAG antibody is human and mouse reactive. |
Application
Western blot analysis of UVRAG in mouse lung tissue lysate with UVRAG antibody at 1 μg/ml.
Application
Immunofluorescence of UVRAG in human lung tissue with UVRAG antibody at 20 μg/ml.
Application
Immunohistochemistry of UVRAG in human lung tissue with UVRAG antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UVRAG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | UVRAG antibody is supplied in PBS containing 0.02% sodium azide. |
