Application
Western blot analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of VAMP7 in human lung tissue with VAMP7 antibody at 20 μg/mL.| Product Name | VAMP7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VAMP7 Antibody: SYBL1, TIVAMP, VAMP-7, TI-VAMP, SYBL1, Vesicle-associated membrane protein 7, Synaptobrevin-like protein 1 |
| Product description | VAMP7 Antibody: VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.1) Galli T, Zahraoui A, Vaidyanathan VV, et al. A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. Mol. Biol. Cell 1998; 9:1437-48. |
| Immunogen | VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7. The immunogen is located within amino acids 20 - 70 of VAMP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VAMP7 |
|---|---|
| Alternative Names | VAMP7 Antibody: SYBL1 TIVAMP VAMP-7 TI-VAMP SYBL1 Vesicle-associated membrane protein 7 Synaptobrevin-like protein 1 |
| Molecular Weight(MW) | Predicted: 24, 29 kDa Observed: 32 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least three isoforms of VAMP7 are known to exist; this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins. |
| Entrez Gene | 6845 |
|---|---|
| Protein Accession | NP_001172112 |
Application
Western blot analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of VAMP7 in human lung tissue with VAMP7 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VAMP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VAMP7 Antibody is supplied in PBS containing 0.02% sodium azide. |
