Application
Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 μg/mL.| Product Name | VARP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VARP Antibody: VARP, PP12899, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein |
| Product description | VARP Antibody: The VPS9 ankyrin repeat protein (VARP) binds to the Rab21, a guanine nucleotide exchange factor that plays an essential role in endocytic trafficking. VARP localizes to early endosomes and is thought to regulate endosome dynamics. VARP also interacts with TI-VAMP/VAMP7, a vesicular SNARE that mediates an exocytic pathway that is crucial to neurite growth. Depletion of VARP by RNA interference impairs neurite growth, suggesting that VARP is a positive regulator of neurite growth.1) Zhang X, He X, Fu XY, et al. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. J. Cell Sci. 2006; 119:1053-62. |
| Immunogen | VARP antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human VARP. The immunogen is located within amino acids 50 - 100 of VARP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| VARP antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANKRD27 |
|---|---|
| Alternative Names | VARP Antibody: VARP PP12899 Ankyrin repeat domain-containing protein 27 VPS9 domain-containing protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (75%) |
Application
Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | VARP antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VARP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VARP Antibody is supplied in PBS containing 0.02% sodium azide. |
