Application
Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.Application
Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.| Product Name | VASH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VASH1 Antibody: KIAA1036, KIAA1036, VASH, Vasohibin-1 |
| Product description | VASH1 Antibody: VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.1) Watanabe K, Hasegawa Y, Yamashita H, et al. Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis. J. Clin. Invest. 2004; 114:989-907 |
| Immunogen | VASH1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1. The immunogen is located within amino acids 290 - 340 of VASH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VASH1 antibody can be used for detection of VASH1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VASH1 |
|---|---|
| Alternative Names | VASH1 Antibody: KIAA1036 KIAA1036 VASH Vasohibin-1 |
| Tissue Specificity | VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform. |
Application
Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.
Application
Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | VASH1 antibody can be used for detection of VASH1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VASH1 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VASH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
