Application
To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.Application
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | Vaspin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OL-64Serpin A12, OL-64, Vaspin |
| Product description | Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography employing immobilized hVaspin matrix |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect hVaspin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. Sandwich To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Vaspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. Western Blot: To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SERPINA12 |
|---|---|
| Alternative Names | OL-64Serpin A12 OL-64 Vaspin |
Application
To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Application
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | ELISA: Indirect: To detect hVaspin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. Sandwich To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Vaspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin. Western Blot: To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Vaspin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
