Application
Western blot analysis of extracts from JK cells using VAV3 (Phospho-Tyr173) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | VAV3 (phospho Tyr173) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VAV3, FLJ40431, Guanine nucleotide exchange factor VAV3 |
| Product description | This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.1) Trenkle T., Nucleic Acids Res. 26:3883-3891(1998). |
| Immunogen | VAV3 (phospho Tyr173) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 173 (E-V-Y (p)-E-D) derived from Human VAV3. |
| Modification | p-Tyr173 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VAV3 |
|---|---|
| Alternative Names | VAV3 FLJ40431 Guanine nucleotide exchange factor VAV3 |
| Molecular Weight(MW) | 100 kDa |
| Tissue Specificity | The antibody detects endogenous levels of VAV3 only when phosphorylated at tyrosine 173. |
| Entrez Gene | 10451 |
|---|---|
| Protein Accession | NP_006104.4 |
Application
Western blot analysis of extracts from JK cells using VAV3 (Phospho-Tyr173) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
