Application
To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.1 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.Application
To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | VCAM-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD106, INCAM-100, L1CAM, Vascular cell adhesion protein 1, V-CAM 1 |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human VCAM-1. Human VCAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCAM1 |
|---|---|
| Alternative Names | CD106 INCAM-100 L1CAM Vascular cell adhesion protein 1 V-CAM 1 |
Application
To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.1 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.
Application
To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | WB:1:500~1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
