Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Product Name | VDAC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VDAC1, PORIN, VDAC-1 |
| Product description | The voltage-dependent anion-selective channel 1 (VDAC1) functions as a channel in membranous structures for the outer mitochondrial membrane, the cell membrane, endosomes, caveolae, the sarcoplasmatic reticulum, synaptosomes, and post-synaptic density fraction. A major function of VDAC1 in the plasma membrane is that of a NADH (-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis.1) Zheng, Y., et al., (2004) Oncogene 23 (6), 1239-1247. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human VDAC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| VDAC1 antibody can be used for detection of VDAC1 by ELISA at 1:312500. VDAC1 antibody can be used for detection of VDAC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | VDAC1 |
|---|---|
| Alternative Names | VDAC1 PORIN VDAC-1 |
| Molecular Weight(MW) | 31 kDa |
Application
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 50ug HeLa lysate, Lane 2: 50ug 293T lysate, Lane 3: 50ug K562 lysate, Lane 4: 50ug MDA-MB-231 lysate).
Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | VDAC1 antibody can be used for detection of VDAC1 by ELISA at 1:312500. VDAC1 antibody can be used for detection of VDAC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store VDAC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
