Application
To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.Application
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.| Product Name | VEGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vpf, Vegf, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF (murine VEGF). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0 2.0 μg/mL of this antibody is required. ELISA: To detect mVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Vegfa |
|---|---|
| Alternative Names | Vpf Vegf Vascular endothelial growth factor A Vascular permeability factor VEGF-A |
Application
To detect mVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.
Application
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5ug/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0 2.0 μg/mL of this antibody is required. ELISA: To detect mVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | VEGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
