Application
To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with ProSci's biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 ug/ml.| Product Name | VEGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vascular Endothelial Growth Factor, VEGF, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A, |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of ELISA: In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VEGFA |
|---|---|
| Alternative Names | Vascular Endothelial Growth Factor VEGF Vascular endothelial growth factor A Vascular permeability factor VEGF-A |
Application
To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with ProSci's biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 ug/ml.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of ELISA: In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
