Application
Western blot analysis of VEZT in 293 cell lysate with VEZT antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.Application
Immunohistochemistry of VEZT in rat kidney tissue with VEZT antibody at 5 μg/ml.| Product Name | VEZT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VEZT Antibody: VEZATIN, Vezatin |
| Product description | Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).1) Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9. |
| Immunogen | VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT. The immunogen is located within amino acids 480 - 530 of VEZT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VEZT |
|---|---|
| Alternative Names | VEZT Antibody: VEZATIN Vezatin |
| Molecular Weight(MW) | Predicted: 63, 68, 78, 81, 83, 86 kDa Observed: 60 kDa |
| Tissue Specificity | VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist. |
Application
Western blot analysis of VEZT in 293 cell lysate with VEZT antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
Application
Immunohistochemistry of VEZT in rat kidney tissue with VEZT antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VEZT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | VEZT antibody is supplied in PBS containing 0.02% sodium azide. |
