Application
Western blot analysis using Vimentin monoclonal antibody against truncated Vimentin recombinant proteinApplication
Immunohistochemical analysis of paraffin - embedded human lung carcinoma tissue showing cytoplasmic location using Vimentin antibody with DAB staining.| Product Name | Vimentin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEL113, CTRCT30 |
| Product description | Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.1) Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996). |
| Immunogen | Ni-NTA purified truncated recombinant Vimentin expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | VIM |
|---|---|
| Alternative Names | HEL113 CTRCT30 |
Application
Western blot analysis using Vimentin monoclonal antibody against truncated Vimentin recombinant protein
Application
Immunohistochemical analysis of paraffin - embedded human lung carcinoma tissue showing cytoplasmic location using Vimentin antibody with DAB staining.| Application Notes | ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Vimentin monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
