Application
Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr821) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Vinculin (phospho Tyr821) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vinculin |
| Product description | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.1) Subauste MC, et al. (2004) J Cell Biol 165, 371-81 |
| Immunogen | Vinculin (phospho Tyr821) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 821 (S-G-Y (p)-R-I) derived from Human Vinculin. |
| Modification | p-Tyr821 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCL |
|---|---|
| Alternative Names | Vinculin |
| Molecular Weight(MW) | 123 kDa |
| Tissue Specificity | The antibody detects endogenous level of Vinculin only when phosphorylated at tyrosine 821. |
| Entrez Gene | 7414 |
|---|---|
| Protein Accession | NP_003364.1 |
Application
Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr821) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
