Application
Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 μg/ml.Application
Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 μg/ml.Application
Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 μg/ml.| Product Name | Vinculin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMD1W, CMH15, Metavinculin, MVCL |
| Product description | Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).1) Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525. |
| Immunogen | Vinculin antibody was raised against a 23 amino acid peptide near the amino of human Vinculin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCL |
|---|---|
| Alternative Names | CMD1W CMH15 Metavinculin MVCL |
| Molecular Weight(MW) | Predicted: 120 kDa Observed: 117 kDa |
| Sequence Similarities | 4) Cat. No. 1202 - Raji Cell Lysate |
| Tissue Specificity | Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist. |
Application
Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 μg/ml.
Application
Immunofluorescence of Vinculin in Jurkat cells with Vinculin antibody at 20 μg/ml.
Application
Immunocytochemistry of Vinculin in Jurkat cells with Vinculin antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Vinculin antibody is supplied in PBS containing 0.02% sodium azide. |
