Application
Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of VISA in Human Brain cells with VISA antibody at 20 μg/mL.Application
Immunohistochemistry of VISA in human brain tissue with VISA antibody at 5 μg/mL.| Product Name | VISA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VISA Antibody: IPS1, VISA, IPS-1, CARDIF, IPS1, KIAA1271, Mitochondrial antiviral-signaling protein, CARD adapter inducing interferon beta, MAVS |
| Product description | VISA Antibody: Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-κB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKα, IKKβ and IKKε kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.1) Seth RB, Sun L, and Chen ZJ. Antiviral innate immunity pathways. Cell Res.2006; 16:141-7. |
| Immunogen | VISA antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human VISA. The immunogen is located within the first 50 amino acids of VISA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAVS |
|---|---|
| Alternative Names | VISA Antibody: IPS1 VISA IPS-1 CARDIF IPS1 KIAA1271 Mitochondrial antiviral-signaling protein CARD adapter inducing interferon beta MAVS |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of VISA in Human Brain cells with VISA antibody at 20 μg/mL.
Application
Immunohistochemistry of VISA in human brain tissue with VISA antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VISA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VISA Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM294952