Application
Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.Application
Immunofluorescence of VLK in rat lung tissue with VLK antibody at 5 μg/mL.Application
Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 μg/mL.| Product Name | VLK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VLK Antibody: Vlk, MAd1, Adtk1, ESTM17, Sgk493, X83346, AI115348, AW548124, Vlk, Protein kinase domain-containing protein, cytoplasmic, Protein kinase-like protein SgK493 |
| Product description | VLK Antibody: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.1) Kinoshita M, Era T, Jakt LM, et al. The novel protein kinase Vlk is essential for stromal function of mesenchymal cells. Development2009; 136:2069-79. |
| Immunogen | VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK. The immunogen is located within amino acids 320 - 370 of VLK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VLK antibody can be used for detection of VLK by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pkdcc |
|---|---|
| Alternative Names | VLK Antibody: Vlk MAd1 Adtk1 ESTM17 Sgk493 X83346 AI115348 AW548124 Vlk Protein kinase domain-containing protein cytoplasmic Protein kinase-like protein SgK493 |
Application
Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.
Application
Immunofluorescence of VLK in rat lung tissue with VLK antibody at 5 μg/mL.
Application
Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | VLK antibody can be used for detection of VLK by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VLK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VLK Antibody is supplied in PBS containing 0.02% sodium azide. |
