Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | VPS24 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VPS24, NEDF, VPS24, CGI-149 |
| Product description | VPS24 encodes a protein that acts in the sorting of transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily.1) Whitley, P., et al., (2003) J Biol Chem. 278(40), 38786-95. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human VPS24. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| VPS24 antibody can be used for detection of VPS24 by ELISA at 1:12500. VPS24 antibody can be used for detection of VPS24 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHMP3 |
|---|---|
| Alternative Names | VPS24 NEDF VPS24 CGI-149 |
| Molecular Weight(MW) | 25 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | VPS24 antibody can be used for detection of VPS24 by ELISA at 1:12500. VPS24 antibody can be used for detection of VPS24 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store VPS24 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
