Application
Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.Application
Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml.Application
Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.| Product Name | VPS39 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VPS39 Antibody: TLP, VAM6, hVam6p, KIAA0770, TLP, TRAP1-like protein |
| Product description | VPS39 Antibody: VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.1) Nakamura N, Hirata A, Ohsumi Y, et al. Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1997; 272:11344-9. |
| Immunogen | VPS39 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39. The immunogen is located within amino acids 710 - 760 of VPS39. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VPS39 antibody can be used for detection of VPS39 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VPS39 |
|---|---|
| Alternative Names | VPS39 Antibody: TLP VAM6 hVam6p KIAA0770 TLP TRAP1-like protein |
| Tissue Specificity | At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1. |
Application
Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.
Application
Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml.
Application
Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | VPS39 antibody can be used for detection of VPS39 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VPS39 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VPS39 Antibody is supplied in PBS containing 0.02% sodium azide. |
