Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
VPS52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of VPS52 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | VPS52 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein, VPS52, SACM2L |
| Product description | This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.1) Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009)Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009) Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008) Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005) Mungall, A.J., et al. Nature 425(6960):805-811(2003) |
| Immunogen | This VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-637 amino acids from the C-terminal region of human VPS52. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VPS52 |
|---|---|
| Alternative Names | Vacuolar protein sorting-associated protein 52 homolog SAC2 suppressor of actin mutations 2-like protein VPS52 SACM2L |
| Molecular Weight(MW) | 82 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
VPS52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of VPS52 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
